See attached example.
Solution
The menubar menuitems JSON string must contain an unique tag per menuitem:
{Text: "_Save", Tag:"FileSave", EventName: "FileSave"},
The menuitems are collated in a global map. The menuitem to change is determined by its tag.
In a subroutine, the menuitem style is changed using the setStyle API menthod (requires the JavaObject library).
Private Sub MenuItemBold(MenuTag As String)
Dim mi As MenuItem = MenuItems.Get(MenuTag)
If mi.IsInitialized Then
Dim joMI As JavaObject = mi
joMI.RunMethod("setStyle", Array("-fx-font-weight: bold;"))
End If
End Sub
In the attached example, menu file > save is set to bold
Hints
If other menuitem types, like a checkmenuiten, then add or change the sub accordingly.
Other styles are also possible, like setting the font to red ("-fx-text-fill:red") etc.